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TUNEYALI Kit (Kit #1000000255)

Depositing Lab : Irina Borodina

TUNEYALI 컬렉션은 Yarrowia lipolytica에서 전사인자 발현을 정밀하게 조절하기 위한 모듈형 toolkit입니다. 

이 키트는 총 64개의 플라스미드로 구성되어 있으며, 그 중 56개는 특정 전사인자의 프로모터 영역을 표적으로 하는 기본 플라스미드이고, 나머지 8개는 다양한 발현 강도를 가진 합성 프로모터를 암호화하는 플라스미드입니다.

이 키트는  96-well plate bacterial glycerol stocks으로 제공됩니다.

Description

The TUNEYALI kit consists of 64 plasmids: 56 base plasmids and 8 plasmids encoding synthetic promoters. Each base plasmid is designed to delete the native promoter of one specific transcription factor among a set of 56, allowing targeted modulation of gene regulation. The synthetic promoters span a range of strengths and can be inserted into the base plasmids using a SapI-based Golden Gate reaction to replace the native promoter.

This modular design enables precise tuning of transcription factor expression by selecting appropriate promoter-plasmid combinations. Moreover, multiple promoters and base plasmids can be assembled simultaneously in a single one-pot SapI-based Golden Gate reaction, enabling efficient construction of a TUNEYALI transcription factor (TF) plasmid library. This high-throughput approach supports systematic studies of TF function, regulatory networks, and synthetic biology applications in Y. lipolytica.

Contents of Kit [Click]